Immune & Longevity · Reconstitution Calculator

Thymosin Alpha-1 reconstitution calculator.

Immune-modulating peptide (brand name Zadaxin) that activates T-cell and NK-cell function. A 5 mg vial in 2 mL bacteriostatic water gives 2.5 mg/mL — the standard clinical dose is 1.6 mg subq, 2× per week.

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Thymosin Alpha-1 · reconstitute with 2 mL BAC water · typical range 1.6–3.2 mg, 2×/weekResearch range. Not medical advice.
1
How much peptide is in the vial?The total mass listed on the label.
2
How much BAC water did you add?Bacteriostatic water injected into the vial.
3
What dose do you want to draw?Per single injection.

Thymosin Alpha-1 dose ranges between 1.6 mg to 3.2 mg (1.6–3.2 mg, 2×/week)

Draw the plunger to 64 units on a U-100 insulin syringe.
That’s 0.640 mL of solution, or 1.6 mg of peptide.

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NEEDLEPLUNGER
Solution will be2.50 mg/mL
Each unit holds25 mcg
You'll inject0.640 mL / 64 units
Vial gives you3 doses
Doses shown are commonly cited research ranges, not medical advice.U-100 · 100 units = 1 mL
Quick reference · Thymosin Alpha-1
5 mg / 2 mL → 2.50 mg/mL
Dose
Volume
U-100 units
Low dose
1.6 mg
0.640 mL
64
Typical dose
1.6 mg
0.640 mL
64
High dose
3.2 mg
1.280 mL
128
Based on a 5 mg vial in 2 mL bacteriostatic water. Typical research range: 1.6–3.2 mg, 2×/week.
★ How to reconstitute

Reconstituting Thymosin Alpha-1, step by step.

  1. 1Draw 2 mL of bacteriostatic water into a 3 mL syringe.
  2. 2Inject slowly into the Thymosin Alpha-1 vial, down the inside wall.
  3. 3Swirl gently — do not shake.
  4. 4Refrigerate. Use within 4 weeks.
★ FAQ

Thymosin Alpha-1 questions, answered.

What's the standard Thymosin Alpha-1 dose?+

1.6 mg subq, twice per week — the dosing used in the FDA-approved Zadaxin protocol for chronic hepatitis B and as a cancer-adjuvant therapy. From a 5 mg / 2 mL mix at 2.5 mg/mL, that's 64 units on a U-100 insulin syringe.

What's Thymosin Alpha-1 actually used for off-label?+

Immune support during chronic infections (Lyme, CMV, EBV reactivation), recovery from acute viral illness, and as an adjunct in autoimmune protocols. Animal data also suggests modest improvements in vaccine response in older adults.

How long do I need to run it?+

Protocols vary. Chronic immune support typically runs 8–12 week cycles followed by a 4-week break. Acute illness protocols are 2–3 weeks at 1.6 mg, 3× per week. Long-term continuous use isn't well characterized in the literature.

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