Metabolic & Longevity · Reconstitution Calculator

NAD+ reconstitution calculator.

Injectable NAD+ bypasses the gut and liver entirely, producing real cellular elevation that oral NMN and NR can't match. A 250 mg vial in 2 mL bacteriostatic water gives 125 mg/mL — most dose 50–500 mg per week.

The Calculator
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NAD+ · reconstitute with 2 mL BAC water · typical range 50–500 mg/weekResearch range. Not medical advice.
1
How much peptide is in the vial?The total mass listed on the label.
2
How much BAC water did you add?Bacteriostatic water injected into the vial.
3
What dose do you want to draw?Per single injection.

NAD+ dose ranges between 50 mg to 500 mg (50–500 mg/week)

Draw the plunger to 100 units on a U-100 insulin syringe.
That’s 1.000 mL of solution, or 125 mg of peptide.

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NEEDLEPLUNGER
Solution will be125.00 mg/mL
Each unit holds1.3 mg
You'll inject1.000 mL / 100 units
Vial gives you2 doses
Doses shown are commonly cited research ranges, not medical advice.U-100 · 100 units = 1 mL
Quick reference · NAD+
250 mg / 2 mL → 125.00 mg/mL
Dose
Volume
U-100 units
Low dose
50 mg
0.400 mL
40
Typical dose
125 mg
1.000 mL
100
High dose
500 mg
4.000 mL
400
Based on a 250 mg vial in 2 mL bacteriostatic water. Typical research range: 50–500 mg/week.
★ How to reconstitute

Reconstituting NAD+, step by step.

  1. 1Draw 2 mL of bacteriostatic water into a 3 mL syringe.
  2. 2Inject slowly down the side of the NAD+ vial.
  3. 3Swirl gently until the solution is clear. Do not shake.
  4. 4Refrigerate. Use within 2 weeks — NAD+ oxidizes faster than most peptides.
★ FAQ

NAD+ questions, answered.

How much BAC water for a 250 mg NAD+ vial?+

2 mL is the standard ratio. That gives you 125 mg/mL — 100 units (1 mL, a full U-100 syringe) is 125 mg, a common weekly maintenance dose.

What's a typical NAD+ dose?+

Low: 50 mg/week. Medium: 125 mg/week. High: 250–500 mg/week. Higher doses are typically split across two shots to fit a single U-100 syringe, or drawn with a larger 3 mL syringe.

Why is reconstituted NAD+ less stable than other peptides?+

NAD+ (reduced form) oxidizes to NADH and NAD+ disulfide rapidly when exposed to air and water. Most protocols freeze single-dose aliquots, or mix only what you'll inject within the next 1–2 weeks.

★ Keep reading

More for NAD+ users.

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